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Addgene inc human fas cytoplasmic domain
Human Fas Cytoplasmic Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fas+cytoplasmic+domain/pGL2-Mcl-1+mut+(Plasmid+%2319133)/pmc03137053-195-13-38
Average 93 stars, based on 4 article reviews

human fas cytoplasmic domain - by Bioz Stars, 2026-09

93/100 stars

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Expressing:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducingwith isopropyl -D-thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 mM EDTA, 10% glycerol, 0.5% aprotinin, 1 mMdithiothreitol, and 1mMPMSF)with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Purification:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Amplification:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Polymerase Chain Reaction:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Clone Assay:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Construct:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Sequencing:

Article Title: Calmodulin Mediates Fas-induced FADD-independent Survival Signaling in Pancreatic Cancer Cells via Activation of Src-Extracellular Signal-regulated Kinase (ERK)
Article Snippet: After inducing with isopropyl β- d -thiogalactoside at 30 °C, the bacteria were lysed in GST lysis buffer (PBS, 50 m m EDTA, 10% glycerol, 0.5% aprotinin, 1 m m dithiothreitol, and 1 m m PMSF) with lysozyme and purified with a GST expression and purification kit (Amersham Biosciences). .. For expression and purification of His-SUMO fusion protein, the cDNA fragment corresponding to human Fas cytoplasmic domain (amino acids 191–335) was amplified from a human umbilical vein endothelial cell library by PCR, or cDNA of Src from pDONR223-Src (Addgene, Cambridge, MA) was cloned into a pET28A-His-SUMO construct (provided by Dr. Jinbiao Ma, University of Alabama at Birmingham) and confirmed by sequencing. ..

Review